New technology enhances CRISPR screening with spatial information

Scientists at the Broad Institute have developed a new technology called Perturb-FISH, which enhances CRISPR screening by incorporating spatial information. This method allows researchers to see how gene changes affect neighboring cells, providing deeper insights into gene functions. Previously, methods like Perturb-seq focused on individual cells without considering their spatial context. Perturb-FISH combines imaging techniques with CRISPR detection, enabling the study of gene interactions and cellular responses in a more comprehensive way. The researchers demonstrated Perturb-FISH's effectiveness in various models, including autism-related gene effects and tumor-immune cell interactions. They aim to refine the method for broader use, increasing the number of genes analyzed and simplifying the process.