New genome editing method corrects multiple mutations at once

Researchers have developed a new genome editing method called prime assembly that can correct multiple genetic mutations at once, as described in a paper published today in Nature. The technique may enable universal gene therapies applicable to many patients. Prime assembly builds on prime editing technology, allowing long DNA fragments to be precisely inserted into targeted genome positions within living cells. Unlike current methods requiring individualized short edits, this single-step process uses DNA flaps as tethers to assemble gene-sized pieces permanently. The targeted approach reduces off-target effects and toxicity risks compared to other methods, and works in nondividing cells. Researchers are now investigating molecular mechanisms to improve efficiency and delivery, aiming for clinical applications in treating inherited diseases.