Researchers optimize gene expression control in CHO cells for membrane proteins

A recent study presented at ELRIG Drug Discovery 2024 explored advanced methods for controlling protein expression in CHO GSKO cells. Researchers tested techniques like Tet-ON systems, stop codon suppression, and IRES elements to achieve precise expression levels of two membrane proteins, Target X and Target Y. The study found that the Tet-ON system allowed for some control over protein expression, but it lacked a dose-dependent response. Stop codon suppression effectively reduced expression levels, while modified IRES elements improved the expression of Protein Y. Overall, four methods were optimized for tuning protein expression. These findings highlight the importance of customizing protein expression for drug discovery projects, particularly for applications requiring specific target levels. The methods developed can be adapted based on project needs and timelines.